Objective:To establish a highly specific liquid chromatography-tandem mass spectrometry(LC-MS/MS)method based on clinical laboratory,so as to quickly and accurately detect serum vitamin A,25 hydroxyvitamin D3[25(OH)D3]and E of population with different ages.Methods:Liquid-liquid extraction pre-process was adopted to establish LC-MS/MS method to detect serum vitamin A,25(OH)D3 and vitamin E.The method was evaluated by matrix effect,linear evaluation,precision measurement and sample recovery rate.A total of 542 specimens were collected from population who received physical examination,and the serum vitamin A,25(OH)D3 and vitamin E levels of different age groups,which included adolescents(1-18 years old group),middle-aged(19-59 years old group)and elderly(≥60 years old group),were analyzed.The nutritional statuses of vitamin A,25(OH)D3 and vitamin E of different populations were judged according to health industry standards(WS/T 553-2017 and WS/T 677-2020).Results:The total detection time was 6.5min when LC-MS/MS method was adopted to simultaneously detect the serum vitamin A,25(OH)D3 and vitamin E,and the obvious interfering factors did not were found.The correlation coefficient(r2)of standard curve was larger than 0.99.The intra batch imprecisions of this method in detecting vitamin A,25(OH)D3 and vitamin E were less than 6.0%,and the inter batch imprecisions were less than 7.5%.The recovery rate was between 100.5%-112.9%.There was no Vitamin A deficiency in 1-18 years old group.In 7-18 years old group,the rate of Vitamin A insufficiency was 8.96%,and the rates of 25(OH)D3 deficiency and insufficiency were respectively 10.12%and 38.10%,and the rate of Vitamin E sufficiency was 5.06%.In 19-59 years old group,the deficiencies of Vitamin A and E were less,and the rates of 25(OH)D3 deficiency and insufficiency were respectively 20.90%and 46.33%.In the"≥60 years old"group,the sums of deficiencies and insufficiencies of vitamin A,25(OH)D3 and vitamin E were respectively 16.36%,87.3%and 12.72%.Conclusion:The established LC-MS/MS technique method can quickly and accurately detect serum vitamin A,25(OH)D3 and vitamin E.There are a certain vitamin A,25(OH)D3 and vitamin E deficiencies in 1-18 years old population and≥60 years old population,and all age groups appear 25(OH)D3 deficiency and insufficiency.
Liquid chromatography tandem mass spectrometry(LC-MS/MS)VitaminNutritional status
国家科技期刊平台
NSTL
万方数据
