Objective:To design a simulated navigation scheme of cranial puncture based on three dimensional (3D) infrared optical locator,so as to achieve accurately puncture of trigeminal nerve semilunar node and ensure maximization of the effect of clinical surgery. Methods:The experiment used computed tomography (CT) to scan skull phantom,and imported its data into 3D Slicer system. Spatial coordinates of skull phantom,surgical tool,surgical needle and needle tips were converted to 3D Slicer through the 3D optical locator and 3D Slicer's image guided therapy (IGT) module,so as to display the relationship of spatial position between needle tip and skull model in real-time,and to verify the accuracy of actual measurement of 3D optical locator,and the located accuracy of navigation scheme of skull puncture. Results:After the verification of 10 verification tests,the defined accuracy of 3D optical locator reached 0.2 mm. The operation of virtual world of 3D Slicer system can real-timely and accurately correspond with that of real world. The measured result of simulated navigation and position was (0.87±0.49) mm. Conclusion:The testing results verify the accuracy of navigation of simulated cranial puncture,which has favorable effect,and which can provide a reference for planning navigation scheme before percutaneous puncture balloon compression (PBC) surgery.