OBJECTIVE:To study the purification and characterization of water soluble polysaccharide JS1 from Hippophae rahamnoides L.fruits that had been extracted with water.METHODS:JS1 was purified by gel chromatography on DEAE-Sep-hadex A-25.its homogeneity was proved by gel chromatogrophy on Sepharose CL-4B glass fiber paper electrophoresis and specific rotator power.Its stral composition was studied by means of GC,IR,etc.RESULTS:JS1 is a water solube polysaccharide.It is consisted of Ara,Xyl,Gal,Glc with the molarity rate of 1∶6∶12∶4 in turn by means of GC.CONCLUSION:JS1 is a neutral heteropolysaccharide.