Isolation, Identification and Antimicrobial Activities of Endophytic Fungi from Cephalotaxus fortunei
OBJECTIVE To isolate and identify endophytic fungi from Cephalotaxus fortunei and to investigage their antibiotic activities, in order to obtain the strains with good antimicrobial activity. METHODS Endophytic fungi were isolated by tissue-culture method and preliminarily identified by morphological method, and the antimicrobial activities of endophytic fungi were determined against six kinds of bacteria by fungus cake method and paper sheet method. RESULTS sixty-one strains of endophytic fungi were isolated from the seed coats, stems and leaves of Cephalotaxus fortunei. By morphological identification, 52 sporulated strains were classified into 9 genera, 5 families and 3 orders. Fifty strains (81. 97% ) showed antimicrobial activity against one or more of the tested microbes, and the secondary metabolites of 40 strains (65. 57% ) presented antibacterial activities against one or more of the tested microbes. CONCLUSION There are abundant and multiple endophytic fungi in Cephalotaxus fortunei, and most of them have strong anti-bacterial activities.
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万方数据
维普
