谱效捕获技术筛选蛇胆陈皮口服液抗炎平喘药效成分
Spectrum Efficiency Capture Technology Screening Anti-Inflammatory and Anti-Asthmatic Components of Shedanchenpi Oral Liquid
虞涛 1万丹 1张瑶纾 1姜民 2董林毅1
作者信息
- 1. 天津医科大学药学院,天津300070
- 2. 南开大学药学院,天津300071
- 折叠
摘要
目的 建立超高效液相色谱/四极杆飞行时间质谱与(NF-κB和β2-肾上腺素能受体)双荧光素酶报告基因检测系统相结合的方法,快速测定蛇胆陈皮口服液中抗炎平喘活性成分.方法 利用NF-κB和β2-肾上腺素能受体活性的双荧光素酶报告基因检测系统,筛选潜在的抗炎解痉成分,同时根据四级杆飞行时间质谱数据对筛选结构进行鉴定.结果 蛇胆陈皮口服液中辛弗林抑制β2-AR激活效应最明显;胆酸、甘氨胆酸、牛磺鹅去氧胆酸和牛磺胆酸抑制NF-κB激活作用最显著.结论 双活性评价谱效捕获方法是对筛选中药复方的有效成分的一种快速有力的手段.
Abstract
OBJECTIVE To develop a simple and dual-target method based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry combined with dual-bioactive (NF-κB and β2-adrenergic receptor) luciferase reporter assay system for rapid determination of various bioactive compounds of the traditional Chinese medicine preparation Shedanchenpi oral liquid.METHODS Potential anti-inflammatory and spasmolytic constituents were screened using NF-κB and β2-adrenergic receptor activity luciferase reporter assay system and simultaneously identified according to the time-of-flight mass spectrometry data.RESULTS Synephrine had the most obvious inhibition on the activation effect of β2-AR.Cholalic acid,glycocholic acid,taurochenodeoxycholic acid,and taurocholic acid activation displayed the most significant inhibitory effect on the activation effect of NF-κB.CONCLUSION Dual bioactivity-integrated online spectrum efficiency capture technology is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines.
关键词
液质联用/β2受体激动剂/抗炎/平喘/双荧光素酶报告基因实验Key words
LC-MS/β2-AR agonist/anti-inflammatory/anti-asthma/dual-luciferase reporter assay引用本文复制引用
基金项目
国家自然科学基金青年项目(81102835)
天津医科大学科研基金(2012KYM012)
出版年
2014
NETL
NSTL
维普
万方数据