OBJECTIVE To isolate,screen and identify the fungi from Aquilaria sinensis (A.sinensis) tree to obtain the fungi which can promote the agarwood formation.METHODS The fungi were isolated by PDA medium,and then inoculate to A.sinensis to verify whether the fungi could promote agarwood formation.The fungi were identified based on morphological characteristics and DNA sequences analysis.RESULTS ASAF05 strain could promote agarwood formation and was identified as P.virgatula,belonging to Pestalotiopsis,Melanconiaceae,Melanconiales,Coelomycetes,and Deuteromycotina.CONCLUSION It is the first reported that P.virgatula can promote agarwood formation in A.sinensis.