柴胡水煎液中柴胡皂苷a和d的溶出与转化差异
Differences Study of Dissolving Behavior and Structure Transformation of Saikosaponin a and d in the Decoction of Radix Bupleuri
李军 1姜华 1张延萍 1周冬菊 1陈丽娜 1张倩1
作者信息
- 1. 河南科技大学化工与制药学院,河南洛阳471023
- 折叠
摘要
目的 考察柴胡皂苷a、d(中药柴胡有效成分,为一对差向异构体,结构相近)在柴胡煎煮过程中的溶出和转化行为.方法 采用HPLC测定柴胡煎煮过程中煎液和药渣在不同时间点各柴胡皂苷的含量变化.色谱条件为WondaSil C18色谱柱(4.6 mm×250 mm,5.m);流动相:甲醇-水(53∶47);流速:1.0 mL·min-;检测波长:210,254 nm.结果 柴胡皂苷a、d在柴胡水煎剂中的溶出和转化行为差异较大.主要体现在:①柴胡水煎液中可以检测到柴胡皂苷a,但检测不到柴胡皂苷d,药渣中柴胡皂苷a和d均能检测到;②无论在煎液还是在药渣中,柴胡皂苷a均少部分降解为柴胡皂苷b1,而柴胡皂苷d在水煎剂中全部转化为柴胡皂苷b2,在药渣中部分转化为柴胡皂苷b2.结论 柴胡水煎液中不存在柴胡皂苷d,取而代之的是其转化产物柴胡皂苷b2.本实验为从水煎剂的角度认识柴胡的有效成分提供参考.
Abstract
OBJECTIVE To study the dissolving behaviors of saikosaponin a and d,a pair of epimeride and the main effective substances in Radix Bupleuri,during the decoction.METHODS HPLC method was employed to determine the contents of saikosaponins in the saiko decoction and its dregs at different decocting time,which was carried out on a WondaSil C18 column (4.6 mm × 250 mm,5 μm) with the mobile phase of methanol-water (53:47) at the flow rate of 1.0 mL · min-1,and the wavelengths were 210 and 254 nm.RESULTS During the decocting process saikosaponin a could be detected in the decoction,however,saikosaponin d could not,which only was found in the dregs of saiko.And saikosaponin a could partly transformed into saikosaponin b1,but saikosaponin d totally changed into saikosaponin b2 in the decoction.Furthermore,in the dreg far less saikosaponin a was transformed than saikosaponind.CONCLUSION In the saiko decoction no saikosaponin d could be detected,but its degradation compound saikosaponin b2 could be found in a large amount.This paper provides clues for the research on the effective substances in the decoction of Radix Bupleuri.
关键词
柴胡/水煎剂/柴胡皂苷/含量测定Key words
Radix Bupleuri/decoction/saikosaponin/content determination引用本文复制引用
基金项目
国家自然科学基金资助项目(81001615)
出版年
2014
NETL
NSTL
维普
万方数据