Simultaneous Determination of Seven Components in Polygoni Multiflori Radix by MEKC-DAD
OBJECTIVE To establish a micellar electrokinetic chromatography with diode array detection (MEKC-DAD) method for simultaneous determination of seven components including stilbene glucoside,emodin,aloe-emodin,rhein,chrysophanol,physcion,and catechin in Polygoni Multiflori Radix.of different origins and commercial herbs.METHODS Based on the mode of MEKC,an uncoated fused silica capillary (75 μm × 64.5 cm,56 cm of effective length) was adopted,and 25 mmol · L-1 borax-30 mmol · L-1 SDS-10％ acetonitrile (pH 9.60) was selected for the running buffer.The detection wavelength was set at 254 nm.The separation voltage was 25 kV and the column temperature was maintained at 24 ℃.The sample was injected at 5 kPa for 5 s.RESULTS The calibration curves of the seven components in Polygoni Multiflori Radix showed good linearity (r≥0.999 4).The average recoveries of the method were between 99.164％-101.504％.CONCLUSION The established method is reliable and accurate,and can be used for the quality control of Polygoni Multiflori Radix.
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万方数据
维普
