Simultaneous Determination of Twenty five Components in the Reference Sample of Banxiabaizhutianma Decoction by UHPLC-MS/MS
OBJECTIVE To establish a UHPLC-MS/MS method for the simultaneous content determination of Adenosine,guanosine,succinic acid,trigonelline,gastrodin,parishin A,parishin B,parishin C,parishin E,atractylenolide Ⅱ,atractylenolideⅢ,chlorogenic acid,neochlorogenic acid,narirutin,hesperidin,rutin,naringin,tangeretin,poricoic acid A,liquiritin,glycyrrhizic acid,liquiritigenin,isoliquiritin,isoliquiritin apioside,cyclic AMP.METHODS The Shim-pack GIST C18(2.1 mm × 100 mm,2μm)column was used,the mobile phase was acetonitrile-0.1％formic acid,gradient elution,flow rate 0.3 mL·min-1;injection volume 1 μL ESI ion source for positive and negative ion scanning analysis using multiple reaction monitoring(MRM)mode.RE-SULTS All of the analytes showed good linearity(r≥0.991)in the tested ranges.The precision,repeatability and stability of the method were good for the twenty five components.The average recoveries were 95.57％-104.98％with the RSDs of 0.55％-4.68％.CONCLUSION This method is strong exclusivity and high sensitivity,which can provide experimental basis for the quality control research of Banxiabaizhutianma Decoction.
Banxiabaizhutianma decoctionbenchmark sampleUHPLC-MS/MScontent determinationquality control
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