Quality Value Transfer of Substance Benchmarks in Qianghuo Shengshi Decoction
OBJECTIVE To establish the fingerprint of the substance benchmark of Qianghuo shengshi decoction(QSD)and the determination method of multi-index composition content,study the transfer rule of the substance benchmark value of QSD,and provide a scientific basis for the subsequent development of QSD and quality control evaluation.METHODS Fifteen batches of QSD sub-stance benchmark samples were prepared according to the method recorded in ancient medical books,and their fingerprint was estab-lished and evaluated,its characteristic peaks was clarified and assigned to the characteristic peaks,and we complete the content deter-mination and transfer rate calculation of the 8 index components in the QSD substance benchmark.RESULTS The similarity of the fingerprint of 15 batches of QSD substance benchmark was between 0.882 and 0.938.A total of 24 common peaks were identified,and 8 common peaks were identified as chlorogenic acid,prim-O-glucosylcimifugin,ferulic acid,senkyunolide Ⅰ,5-O-methylvisammioside,ammonium glycyrrhizinate,notopterygol,osthole.The paste yield of 15 batches of QSD was 22.54％-29.92％,and the average paste transfer rate was 70.69％.The content and average transfer rate of each index component from decoction pieces to substance bench-marks were 0.077 2％-0.106 6％and 128.05％for chlorogenic acid,prim-O-glucosylcimifugin 0.015 5％-0.023 8％and 120.26％,ferulic acid 0.047 5％-0.076 0％and 114.49％,senkyunolide Ⅰ 0.006 6％-0.008 9％and 132.93％,5-O-methylvisam-mioside 0.007 2％-0.009 9％and 90.27％,ammonium glycyrrhizinate 0.181 6％-0.273 5％and 80.53％,notopterygol 0.000 7％-0.001 3％and 50.97％,osthole 0.000 9％-0.002 2％and 13.67％.CONCLUSION In this experiment,a combination of finger-print,paste yield and multi-index composition content determination could be used to analysis the transfer rate of QSD decoction pieces to substance benchmarks,and provide a method reference for the quality control and subsequent development of this prescription.
NETL
NSTL
万方数据
