摘要
目的 研究辣木叶乙酸乙酯部位的化学成分,并初步评价其抗氧化活性.方法 运用各种色谱技术对辣木叶乙酸乙酯部位的化学成分进行分离和纯化,并依据质谱、核磁共振等波谱数据鉴定化合物的结构;采用2,2-联苯基-1-苦基肼基(DPPH)和2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)法测试各化合物的体外抗氧化活性.结果 从辣木叶乙酸乙酯部位中分离得到 14 个化合物,分别为淫羊藿次苷 B1(1)、(3S)-O-β-D-glucopyranosyl-6-[3-oxo-(2S)-butenylideny]-1,1,5-trimethylcyclohex-an-(5R)-ol(2)、淫羊藿次苷 B2(3)、9-hydroxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside(4)、鸢尾苷元(5)、鸢尾苷(6)、鸢尾甲苷A(7)、鸢尾甲苷B(8)、5-羟基-2-羟甲基吡啶(9)、草夹竹桃苷(10)、3,4,5-三甲氧基-1-O-β-D-苯酚葡萄糖苷(11)、1-0-(4-羟甲基苯基)α-L吡喃鼠李糖苷(12)、苄基-O-β-D-葡萄糖苷(13)以及 methyl 2-[4-(α-L-rhamnopyranosyl)phenyl]acetate(14).其中化合物6、7和8具有一定的抗氧化活性,但均弱于阳性对照维生素C的活性.结论 化合物2～11为首次从辣木属植物中分离得到.
Abstract
OBJECTIVE To study the chemical constituents of ethyl acetate extract from Moringa oleifera leaves.METHODS The chemical constituents of ethyl acetate extract from Moringa oleifera leaves were isolated and purified by chromatographic methods.The structures of isolated compounds were identified by MS and NMR data.DPPH and ABTS were used to test the antioxidant activities of the compounds in vitro.RESULTS Fourteen compounds were isolated from ethyl acetate extract of Moringa oleifera leaves and identified as icariside B1(1),(3S)-O-β-D-glucopyranosyl-6-[3-oxo-(2S)-butenylideny]-1,1,5-trimethylcyclohexan-(5R)-ol(2),icariside B2(3),9-hydroxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside(4),tectorigenin(5),tectoridin(6),iristectorin A(7),iris-tectorin B(8),5-hydroxy-2-hydroxymethylpyridine(9),androsin(10),3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside(11),1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside(12),benzyl-O-β-D-glucopyranoside(13)and methyl 2-[4-(α-L-rhamnopyrano-syl)phenyl]acetate(14),respectively.Among them,compounds 6,7 and 8 had certain antioxidant activities,but the activities were weaker than that of vitamin C as positive control.CONCLUSION Compounds 2-11 are isolated from Moringa genus for the first time.
基金项目
北京中医药大学纵向科研发展基金资助(2022-ZXFZJJ-001)
NETL
NSTL
万方数据