基于网络药理学和HPLC-MS/MS分析的参松养心胶囊药效物质筛选及含量测定
Screening and Determination of Effective Components in Shensong Yangxin Capsules Based on Network Pharmacology and HPLC-MS/MS Analysis
刘敏彦 1何敬宇 1何庆国 2董岩 1张会欣 1苏红宁3
作者信息
- 1. 石家庄学院,河北省藏药质量技术创新中心,石家庄 050035;石家庄市靶点药物研究与药效学评价重点实验室,石家庄 050035
- 2. 河北宇辰医药科技有限公司,石家庄 050035
- 3. 石家庄市第四医院,石家庄 050011
- 折叠
摘要
目的 本研究拟构建"成分-靶点-疾病"相关联的多维网络系统,发现参松养心胶囊中与功效相关的质量标志物,并以发现的质量标志物为评价指标,建立高效快速的含量测定方法.方法 采用网络药理学筛选的药效物质作为质量标志物,采用液质联用技术,多反应监测和正负离子切换分段检测模式进行含量测定;色谱柱为Waters C18色谱柱,流动相为体积分数0.1％甲酸水和乙腈,梯度洗脱,流速为0.8 mL·min-1.结果 根据网络药理学研究结果,选择贡献度较高的20种化合物作为质量标志物进行含量测定,方法学验证结果表明20种被测化合物在检测范围内均具有良好的线性关系(r2＞0.991 1),精密度、准确度和稳定性均良好.结论 本研究建立的方法简单、快速、准确可靠,能够用于参松养心胶囊的药效物质筛选和质量评价.
Abstract
OBJECTIVE To construct a multi-dimensional network system of"component-target-disease",find the quality mark-ers related to the efficacy of Shensong Yangxin capsules,and establish an efficient and rapid determination method.METHODS The pharmacodynamic substances selected by network pharmacology were used as quality markers and a novel HPLC-ESI-MS/MS method was developed.During the development of the method,multiple-reaction monitoring scanning and a multi-periods program were applied for quantification by switching electrospray ion source polarity between positive and negative modes at appropriate time points in a single run.Chromatographic separation was performed on a Waters C18 column with linear gradient elution of 0.1％formic acid aqueous solu-tion and acetonitrile at a flow rate of 0.8 mL·min-1.RESULTS According to the results of network pharmacology,twenty com-pounds with high contribution were selected as quality markers for content determination.The results of methodological verification showed that all calibration curves had good linearity(r2＞0.991 1)within the test ranges.The precision,accuracy and stability of the method were all satisfying.CONCLUSION The analytical method is simple,rapid,specific and reliable,which is useful for phar-macodynamic substance screening and comprehensive quality evaluation of Shensong Yangxin capsules.
关键词
网络药理学/高效液相色谱串联质谱/参松养心胶囊/多反应监测/正负离子转换模式Key words
network pharmacology/HPLC-MS/MS/Shensong Yangxin capsule/MRM/polarity switching technology引用本文复制引用
基金项目
河北省自然科学基金资助(H2022106048)
石家庄学院博士科研启动基金资助(22BS001)
出版年
2024
NETL
NSTL
万方数据