Screening and Determination of Effective Components in Shensong Yangxin Capsules Based on Network Pharmacology and HPLC-MS/MS Analysis
OBJECTIVE To construct a multi-dimensional network system of"component-target-disease",find the quality mark-ers related to the efficacy of Shensong Yangxin capsules,and establish an efficient and rapid determination method.METHODS The pharmacodynamic substances selected by network pharmacology were used as quality markers and a novel HPLC-ESI-MS/MS method was developed.During the development of the method,multiple-reaction monitoring scanning and a multi-periods program were applied for quantification by switching electrospray ion source polarity between positive and negative modes at appropriate time points in a single run.Chromatographic separation was performed on a Waters C18 column with linear gradient elution of 0.1％formic acid aqueous solu-tion and acetonitrile at a flow rate of 0.8 mL·min-1.RESULTS According to the results of network pharmacology,twenty com-pounds with high contribution were selected as quality markers for content determination.The results of methodological verification showed that all calibration curves had good linearity(r2＞0.991 1)within the test ranges.The precision,accuracy and stability of the method were all satisfying.CONCLUSION The analytical method is simple,rapid,specific and reliable,which is useful for phar-macodynamic substance screening and comprehensive quality evaluation of Shensong Yangxin capsules.
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