Anti-Alcohol and Liver-Protecting Effects of Four Ancient Prescriptions and the Potential Mechanisms
OBJECTIVE To compare the anti-alcohol and liver-protecting effects of four ancient prescriptions,Baibei powder,Shigao decoction,Jupi decoction and Gegen powder,and explore the potential mechanisms of the four ancient prescriptions in the treat-ment of alcoholic liver disease(ALD).METHODS Rats were randomly divided into normal group,model group and drug administration groups,and the rat drunken model was prepared with 56° liquor.The rats in each administration group were given Chinese medicine decoction by gavage,and the normal group and the model group were given pure water by gavage.The drunken sleep time of rats was recorded,and the blood ethanol concentration and the activities of alcohol dehydrogenase(ADH)and aldehyde dehy-drogenase(ALDH)in serum and liver were measured.Network pharmacology method was used to analyze the effective components,key targets and signaling pathways of the four ancipent prescriptions in the treatment of ALD.RESULTS The four ancient prescrip-tions could significantly prolong the drunkenness time and shorten the sobering time of drunken model rats.Among them,Baibei pow-der could significantly increase the serum activities of ADH,ALDH and super-oxide dismutase(SOD)and improve triglyceride(TG)and glutathione(GSH)contents(P＜0.05).Shigao decoction could significantly reduce the content of serum TG.Jupi decoction could significantly improve the serum GSH and malondialdehyde(MDA)content.Gegen powder could significantly reduce the concen-tration of blood ethanol,improve the activities of ADH,ALDH and SOD and the contents of TG,total cholesterol(TC),glutathione S-transferase(GST),GSH and MDA in the serum or liver(P＜0.05 or P＜0.01).The four prescriptions could reduce the degree of liver pathological damage and reduce the area of lipid droplets to varying degrees.Through the network pharmacology analysis,a total of 78 targets were screened out for the treatment of ALD by the four prescriptions.Among them,tumor necrosis factor(TNF),protein kinase B1(AKT1),estrogen receptor 1(ESR1),epidermal growth factor receptor(EGFR),cytochrome P450 2C19(CYP2C19)and peroxisome proliferator activated receptoα(PPARα)could be used as important targets for the treatment of ALD.Kyoto encyclopedia of genes and gnomes pathway enrichment analysis showed that the ancient prescriptions may play a pharmacodynamic role through PPAR,AMP-activated protein kinase(AMPK),TNF/PPARα-CYP2E1-ALDH signaling pathway.CONCLUSION The four pre-scriptions all have certain anti-alcohol and liver protection effects,and the effect of Gegen powder is more significant.The four pre-scriptions may regulate key targets such as TNF,AKT1,cytochrome P450 2E1(CYP2E1),PPARα and ALDH by acting on the TNF/PPARα-CYP2E1-ALDH pathway,thereby accelerating alcohol metabolism,improving lipid metabolism,and reducing oxidative stress.
Jiejiu prescriptionnetwork pharmacologyanti-alcohol and liver protectionaction mechanism
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