细胞膜色谱与线粒体膜色谱技术的发展及应用
Development and Application of Cell Membrane Chromatography and Mitochondrial Membrane Chroma-tography Technology
赵壮 1范强 1苏武 1陈乐融 1李华 1葛帅 2孔宇 1龙建纲1
作者信息
- 1. 西安交通大学,生命科学与技术学院,线粒体生物医学研究所,生物医学信息工程教育部重点实验室,西安 710049
- 2. 西安交通大学,药学院,西安 710049
- 折叠
摘要
细胞膜色谱法是近年来迅速发展的一种生物亲和色谱方法.其以包覆含受体膜的载体为固定相,将配体与膜受体间相互作用转化为色谱过程.该方法具有生物仿生、高效、低成本等优势,已被广泛用于药物分子筛选和受体与配体相互作用研究.线粒体作为重要的细胞内膜系统,膜结构中分布有多种药物靶点,线粒体膜色谱技术近期也有重要研究进展.笔者综述了细胞膜色谱技术及其应用方面的进展,也对基于细胞膜色谱理论的线粒体膜色谱技术进行简介和展望.
Abstract
Cell membrane chromatography is a bioaffinity chromatography method which has been developing rapidly in recent years,where the carriers coated with receptor-containing biomembrane are packed to stationary phase and the bioaffinity between com-pound and membrane receptor presents in chromatography process.This method has the advantages of biomimetic,high efficiency and low cost,and has been widely used in drug screening and identifying the affinity between membrane receptor and candidate ligand.Mitochondria,a crucial functional intracellular membrane system,with various drug targets residing in membrane.Recently,signifi-cant research advancements have been made in mitochondrial membrane chromatography technology.In this paper,the recent progres-ses and applications of cell membrane chromatography and mitochondria membrane chromatography are reviewed.
关键词
细胞膜色谱/线粒体膜色谱/药物筛选/药物受体相互作用Key words
cell membrane chromatography/mitochondrial membrane chromatography/drug screening/drug-receptor interaction引用本文复制引用
基金项目
载人航天工程航天医学实验领域项目资助(HYZHXM01023)
出版年
2024
NETL
NSTL
万方数据