OBJECTIVE To screen one Monascus purpureus stain that has high yield of lovastatin and provide potential strains for the development of red yeast rice(RYR)industry.METHODS Twelve RYR samples were collected,and Monascus sp.was isolated from these samples using traditional isolation technology and identified by morphology and ITS sequencing.All M.purpureus strains were screened and their total lovastatin contents[including lactone lovastatin(C24H36O5)and acid lovastatin(C24H38O6)]were deter-mined by high performance liquid chromatography(HPLC).Soft SPSS 25.0 was used for variance analysis.RESULTS A total of 35 Monascus sp.strains were isolated and 23 of them were identified at specie level,including 11 M.purpureus strains,10 M.ruber strains and 2 M.aurantiacus strains.There was significant difference(P＜0.05)in lovastatin production among 11 M.purpureus strains.Among them,SM-1 isolate produced the highest amount of total lovastatin,with the value reaching(4.652±0.001)mg·g-1,followed by M4-1,F1-3,and F5-3 strains with the value reaching(2.903±0.033)and(3.108±0.009)mg·g-1,re-spectively.M4-5 isolate produced the lowest amount of lovastatin with the value of(2.712±0.014)mg·g-1.CONCLUSION Fungal strain is chaotic in the fermentation of RYR on the market.A high-yielding lovastatin strain SM-1 is screened and can be used a potential strain for RYR production.
Monascus sp.isolation and molecular identificationlovastatinstrain screening
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