首页|新疆软紫草有效成分分离及保肝抗HBV作用研究

新疆软紫草有效成分分离及保肝抗HBV作用研究

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
目的 研究新疆软紫草(AER)石油醚部位(AERP)及水溶性部位(AERW)对小鼠急性肝损伤的保护作用，并对效果较好的石油醚部位进行有效成分分离，研究其有效成分抗乙型肝炎病毒(HBV)作用。方法 C57小鼠48只，随机分为6组，给药14 d后，在末次2 h采用脂多糖/D-氨基半乳糖(LPS/D-Galn)诱导制备炎症浸润性急性肝损伤模型，测定肝脾指数、小鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)含量以及肝组织匀浆中的NO含量，并进行肝组织病理切片的考察。采用正相硅胶，反相硅胶，薄层制备板对石油醚提取物进行分离，得到4个萘醌类有效成分，采用MTT法检测4个萘醌类有效成分对HepG2。2。15细胞的抗乙型肝炎病毒活性。结果 AERW高剂量组和AERP低剂量组均可不同程度降低小鼠血清中的AST、ALT活性以及肝组织中的NO含量，并使肝组织病变不同程度地得到改善，对比分析后AERP具有更好的保肝抗炎作用，之后对AERP的提取方法进行优化，得到含有效成分更充分的AERP，并分离得到4个萘醌类有效成分:异戊酰紫草素、乙酰紫草素、2，3-二甲基戊烯酰紫草素和紫草素。4个萘醌类化合物对HepG2。2。15细胞均具有较好的抑制作用。结论 研究表明，AERW及AERP对急性肝损伤具有较好的保护作用，AERP的保肝效果优于AERW，AERP的4种萘醌类有效成分对HBV具有较好的抑制作用。综上所述，AER提取物具有显著的保肝作用，并且AER的有效成分对乙型肝炎病毒具有显著的抑制作用。
Antiinflammation and Antihepatitis B Virus Effects of Extracts from Arnebia euchroma(Royle)Johnst
OBJECTIVE To study the protective effect of petroleum ether fraction(AERP)and water soluble fraction(AERW)of Arnebia euchroma(Royle)Johnst.on acute liver injury in mice,and to isolate the effective components of petroleum ether fraction with better effect,and to study the antihepatitis B virus effect of its effective components.METHODS Forty eight C57 mice were randomly divided into 6 groups.After 14 days of administration,lipopolysaccharide/D-galactosamine(LPS/D-Galn)was used to induce acute liver injury with inflammatory infiltration at the last 2 hours.The liver and spleen index,the content of AST and ALT in mouse serum and the content of NO in liver homogenate were measured,and the pathological section of liver tissue was investigated.The petroleum ether extract was separated by normal silica gel,reverse silica gel and thin layer preparation plate,and four active naphthoquinones were obtained.The antihepatitis B virus activity of the four active naphthoquinones on HepG2.2.15 cells was detected by MTT meth-od.RESULTS Both AERW high-dose group and AERP low-dose group can reduce the activity of AST and ALT in serum and the con-tent of NO in liver tissue of mice to varying degrees,and improve the pathological changes of liver tissue to varying degrees.After com-parative analysis,AERP has better liver protective and anti-inflammatory effects.The extraction method of AERP was optimized to obtain AERP with more effective components,and four active naphthoquinones were isolated:isovalerylshikonin,acetylshikonin,2,3-dimethyl-pentenylshikonin and shikonin.Four active naphthoquinones have good inhibitory effects on HepG2.2.15 cells.CONCLUSION The results show that AERW and AERP have a good protective effect on acute liver injury.The liver protective effect of AERP is better than that of AERW.The four active components of AERP have a good inhibitory effect on HBV.AER extract has a significant liver protective effect,and the active components of AER have a significant inhibitory effect on hepatitis B virus.

Arnebia euchroma(Royle)Johnst.anti hepatitis B virusacute liver injurycomponent extractioncomponent isola-tionHepG2.2.15 cell

孟凌宇、吕博、侯金秋、潘培妍、杜梦鸽、田星、秦冬梅

展开 >

石河子大学药学院/新疆植物药资源利用教育部重点实验室,新疆石河子 832002

石河子大学医学院第一附属医院,新疆石河子 832002

新疆软紫草 抗乙型肝炎病毒 急性肝损伤 提取分离 HepG2.2.15细胞

国家自然科学基金资助新疆生产建设兵团重大科技项目资助新疆植物药资源利用教育部重点实验室开放课题资助

818607302020AA005201801

2024

10.11669/cpj.2024.03.007

中国药学杂志
中国药学会

中国药学杂志

CSTPCD北大核心
影响因子：0.957
ISSN：1001-2494
年,卷(期)：2024.59(3)
  • 19