Analysis of Absorbed Components of Rhizoma Anemarrhenae-Cortex Phellodendri Chinesis Herb-Pair Based on UPLC-Q-Exactive Orbitrap-MS
OBJECTIVE To study the components of Rhizoma Anemarrhenae-Cortex Phellodendri Chinesis(RA-PC)herb-pair absorbed into blood in normal rabbits.METHODS Rabbits were fed with the decoction of RA-PC herb pair(4.5 g·kg-1)once a day for 7 days,then blood samples were taken.High-performance liquid chromatography-quadrupole-electrostatic field orbital trap(UPLC-Q-Exactive Orbitrap-MS)technique was applied for the analysis,and fragments were split in both positive and negative ion modes.The compositions into blood were determined according to retention time,accurate relative molecular mass,and secondary mass spectra.RESULTS A total of 54 components were identified in the drug-containing serum of rabbits,including 46 prototype compo-nents,which were alkaloids,flavonoids,saponins,glycosides,phenolic acids,phenylpropanoids,lactones,and phenolic derivatives.The nine metabolites were derived from berberine,jatrorrhizine,berberrubine,and mangiferin,respectively.CONCLUSION RA-PC contains 46 chemical components that can enter blood of normal rabbits,and may also contains four chemical components which are metabolized into nine metabolites.
万方数据
维普
