基于多元统计分析评价不同干燥方法对金钱草中多元指标成分的影响
Evaluation of Effects of Different Drying Methods on Multiple Index Constituents in Lysimachiae Herba Based on Multivariate Statistical Analysis
周永逸 1陈海杰 1薛佳 1邹立思 1吴楠 1袁嘉欢 1刘训红 2蔡芷辰 1陈翠花 1杨薇 1程建明2
作者信息
- 1. 南京中医药大学药学院,南京 210023
- 2. 南京中医药大学药学院,南京 210023;江苏省经典名方工程研究中心,南京 210023
- 折叠
摘要
目的 探究不同干燥方法对金钱草中多元指标成分的影响.方法 采用超高效液相色谱-三重四极杆/线性离子阱质谱(UHPLC-QTRAP-MS/MS)同时测定不同干燥方法下金钱草中黄酮、有机酸、氨基酸及核苷类共44种指标成分的含量;根据44种目标成分的含量,用聚类分析(HCA)、灰色关联度分析(GRA)及逼近理想解排序方法(TOPSIS)法对不同干燥方法下金钱草样品进行综合评价.结果 HCA结果显示,7个不同干燥方法主要分为2类,第1类为晒干和阴干,第2类为不同温度烘干、微波干燥和冷冻干燥;综合GRA和TOPSIS结果,晒干的金钱草样品综合质量较好,阴干和冷冻干燥也可以作为代替干燥方法.结论 该研究可为揭示金钱草传统干燥方法科学内涵提供依据,同时为金钱草药材产地加工时干燥方法的优选提供基础资料.
Abstract
OBJECTIVE To explore the effects of different drying methods on multiple index constituents in Lysimachiae Herba.METHODS A comprehensive analytical method employed with ultra high performance liquid chromatography with triple quadrupole linear ion trap mass spectrometry(UHPLC-QTRAP-MS/MS)was used for simultaneous determination of forty-four constituents inclu-ding flavonoids,organic acids,amino acids,and nucleosides in Lysimachiae Herba.The hierarchical cluster analysis(HCA),gray relation analysis(GRA),and technique for order preference by similarity to ideal solution(TOPSIS)methods were applied to compre-hensively analyze and evaluate the results of multiple index constituents.RESULTS HCA results showed that seven different drying methods were mainly divided into two categories,the first category was sun drying and shady drying,and the second category was drying at different temperatures,microwave drying,and freeze drying.In addition,GRA and TOPSIS results showed that sun drying samples had better quality,and shady drying and freeze drying could be used as alternative drying methods.CONCLUSION This study can provide a basis for revealing the scientific connotation of traditional drying method of Lysimachiae Herba,and provide information for the optimiza-tion of drying method during the processing of Lysimachiae Herba.
关键词
金钱草/干燥方法/超高效液相色谱-三重四极杆/线性离子阱质谱/多元指标成分/多元统计分析Key words
Lysimachiae Herba/drying method/UHPLC-QTRAP-MS/MS/multiple index constituent/multivariate statistical analysis引用本文复制引用
基金项目
江苏高校品牌专业建设工程项目(PPZY2015A070)
出版年
2024
NETL
NSTL
万方数据