Establishment and Application of TaqMan Real-Time PCR Method for Periplaneta americana
OBJECTIVE To establish a highly specific TaqMan real-time PCR method for the identification of Periplaneta ameri-cana medicinal materials and Periplaneta americana powder.METHODS According to the characteristics of genome sequence of Periplaneta americana,specific primers and probes suitable for real-time PCR detection were designed,and the probe concentration,cycle times and annealing temperature were optimized to establish the best TaqMan real-time PCR method of Periplaneta americana.RESULTS The specific primers and probes were only specifically amplified from Periplaneta americana medicinal materials and Periplaneta americana powder,and no fluorescence curves were amplified from Blaptica dubia,Eupolyphaga Sinensis W.Blattella ger-manica and Aspongopus chinensis Dallas.CONCLUSION The established TaqMan real-time PCR method can accurately and rapidly identify Periplaneta americana and common counterfeit products,providing a more specific technical reference for the identification of Periplaneta americana.
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