Ginkgo Flavone Treats Androgenic Alopecia in Mice by Regulating the Expression of VEGF and TGF-β
OBJECTIVE To investigate the effect and mechanism of ginkgo flavone on androgenetic alopecia(AGA)in mice.METHODS AGA model of mice was established by applying 1 mg·mL/-1(mass concentration)testosterone solution after hair removal in blank group,model group,positive drug group and drug administration group.The positive drug group was coated with 50 mg·mL-1(mass concentration)minoxidil,and the administration group was coated with 5％,10％and 20％(mass fraction)ginkgo flavone solution,respectively.The hair growth rates of mice in the hair removal area on day 28 were observed.The hair quali-ties and numbers of hair follicles were observed by scanning electron microscopy(SEM)and HE staining.The levels of vascular endo-thelial growth factor-A(VEGF-A)and transforming growth factor-β(TGF-β)in skin were detected by immunohistochemistry(IHC)and Western blot(WB),and the levels of testosterone,dihydrotestosterone,estrogen,and ROS in the skin tissue were detected by ELISA.RESULTS Compared with the model group,ginkgo flavone could significantly accelerate the growth rate of hair in AGA model mice,increase the number of hair follicles,and increase the length,diameter and scale size of regenerated hair.The mechanism study found that ginkgo flavone could increase the level of VEGF-A in the skin of mice,reduce the level of hair growth inhibitor TGF-β,and had a certain regulatory effect on the level of sex hormones in the skin of mice,while the model under this experimental condi-tion may not be related to oxidative stress.CONCLUSION Ginkgo flavone can promote hair regeneration and improve the quality of regenerated hair in AGA model mice,and its mechanism may be related to promoting hair follicle regeneration by reducing the level of androgens in the skin,decreasing the expression of TGF-β,and increasing the expression of VEGF-A.
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