OBJECTIVE An efficient and stable HPLC-LTQ-Orbitrap-MS/MS method was developed to analyze and identify the chemical constituents from Tripterospermum chinense(Migo)H.Smith rapidly.METHODS The chemical components in T.chinense were analyzed by high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry(HPLC-LTQ-Orbitrap-MS/MS).HPLC-LTQ-Orbitrap-MS/MS was used to analyze the chemical components in T.chinense quickly.The accurate molecular weight,reference substance and multistage fragment ion information of the compounds obtained by HPLC-LTQ-Orbitrap-MS/MS scan-ning,combined with relevant hterature and fragmentation regularity,were used to achieve the accurate qualitative analysis of T.chinense.RESULTS A total of 92 chemical constituents were identified,including flavonoids,terpenoids,organic acids,anthra-quinones,saccharide,ester and the others.Among them,compounds 3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromeh-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7oS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid and Glc-Glc-octadecatrienoyl-sn-glycerol were identified for the first time in this plant by GNPS database alignment.CONCLUSION A rapid qualitative analysis method of chemical components in T.chinense is established,which provides a scientific basis for the extraction and isolation of the chemical constituents of T.chinense and its pharma-codynamic material basis,and provides a certain reference significance for its quality evaluation and application development.
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