Analysis of Different Components and Their Associated Safety Evaluation of Succus Bambusae Prepared by Dry Distillation Process
OBJECTIVE To compare the differences in the chemical compositionof Succus Bambusae with different processes,analyze the safety of Succus Bambusae produced by dry distillation from theperspectives of acuteand subacute toxiciy and provide data support for the further improvement ofthe process standardization andquality standards ofthe large variety offresh bamboo distillate.METHODS Using GC-MS method,combined with PCA and OPLS-DA mathematical algorithms,the differential components and u-nique components among the three processes were analyzed,and the HPLC method was used to quantitatively analyze the indicators re-lated to toxicity potential.Toxic potential was assessed using a combined acute and subacute toxicity protocol.RESULTS Relative content and substance types have changed significantly,total of 47 components were identified from different processes of Succus Bam-busae,including 16 phenols,11 ketones,9 esters,7 aldehydes,3 carboxylic acids,and 1 alcohol.The results of quantitative analysis of phenol and guaiacol showed that only phenol and guaiacol were detected in the dry distillation process,and the contents were(0.097±0.004)and(0.150±0.017)mg·mL-1 respectively.The results of acute toxicity test showed that dry distillation of Succus Bambusae decoction had adverse effects on the body weight and gastrointestinal tract of mice.The subacute toxicity test results are con-sistent with the acute toxicity test results.The high-dose group of dry-distilled Succus Bambusae had adverse effects on the gastrointesti-nal tract tissue,and the organ indexes of heart,liver,lung and kidney were also significantly increased,but no adverse effects appeared in the low-dose and middle-dose groups.CONCLUSION The huge difference in the material basis of dry distillation Suc-cus Bambusae does have safety risks,and the correlation analysis shows that the risk factors are irritating phenolic components such as phenol and guaiacol.However,the safety evaluation indicates that dry distillation of Succus Bambusae is generally safer.This paper could provide toxicological data support for the clinical application of Succus Bambusae and its preparations.
万方数据
维普
