Analysis of the Inhibitory Effect of Cordycepin on Shear-Induced Platelet Aggregation and Activation by Microfluidic Technique
OBJECTIVE To observe the inhibitory effect of cordycepin on platelet aggregation and activation induced by different shear rates.METHODS Polydimethylsiloxane(PDMS)-glass microchannel chips were fabricated by soft lithography.The whole blood of normal people anticoagulated with sodium citrate was collected and incubated with different concentrations of cordycepin in vitro,the blood flowed through the straight microchannel or channel with 80％narrow for 150 seconds at the speed of 14.7 μL·min-1 and 50 µL·min-1 respectively.The adhesion and aggregation images of fluorescent labeled platelets on glass surface were photo-graphed with the microscope,and the fluorescent images were analyzed with Image J.The platelet surface coverage percent was used as a quantitative index of platelet aggregation behavior.The effect of cordycepin on platelet calcium mobilization and monocyte-platelet aggregate(MPA)was analyzed by flow cytometry.The risk of cordycepin was assessed through test of blood coagulation.RESULTS Cordycepin inhibits platelet aggregation and the inhibition effect is related to the shear rates.At 14.7 and 50 μL· min-1,platelet aggregation can be inhibited by cordycepin.Cordycepin inhibits platelet calcium mobilization and MPA effectively.It has no effect on exogenous and endogenous coagulation pathways.CONCLUSION Cordycepin can effectively inhibit shear-induced platelet aggregation and is a potential antiplatelet drug.
万方数据
维普
