首页|胶膜菌属真菌S7糖类成分对铁皮石斛种子萌发的影响

胶膜菌属真菌S7糖类成分对铁皮石斛种子萌发的影响

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目的 研究胶膜菌属真菌S7(Tulasnella sp.)糖类成分对铁皮石斛种子萌发的影响.方法 通过水提醇沉法制备S7菌丝多糖和发酵液多糖.三氟乙酸水解制备菌丝多糖和发酵液多糖的水解物.利用柱前衍生高效液相色谱法(PMP-HPLC)测定多糖的单糖组成.在水琼脂和1/2 MS培养基中分别添加菌丝多糖及其水解物,发酵液多糖及其水解物,以及菌丝多糖特有单糖组分和混合单糖组分,研究S7糖类成分对铁皮石斛种子萌发的影响.结果 水琼脂培养基对照组(WACK1)萌发率(88.65± 4.71)%,种子能萌发至3级(原球茎).与WACK1相比,添加菌丝多糖及其水解物对萌发率没有显著影响(P>0.05),但种子能萌发至4级(1片叶);添加发酵液多糖水解物能显著降低萌发率(P<0.05).菌丝多糖由葡萄糖、半乳糖、葡萄糖醛酸、木糖、甘露糖、岩藻糖、核糖和阿拉伯糖组成,摩尔比为15.05∶1.00∶0.70∶0.35∶0.30∶0.29∶0.13∶0.13.发酵液多糖由阿拉伯糖、半乳糖、木糖和葡萄糖醛酸组成,摩尔比为1.22∶1.00∶0.71∶0.47.岩藻糖是菌丝多糖特有的单糖组分.1/2 MS培养基对照组(MSCK)萌发率和5级萌发率(2片叶)分别为(98.10±0.46)%和(50.97±4.33)%.与MSCK相比,添加0.20~20 mg.L-1岩藻糖对萌发率没有显著影响(P>0.05),但5级萌发率有显著提高(P<0.05),是MSCK的1.36~1.47倍.结论 S7菌丝体多糖及其水解物具有促进铁皮石斛原球茎发育的作用,岩藻糖是S7菌丝多糖水解物中促进种子萌发后进一步分化发育的活性成分.
Effects of Carbohydrate Components of Fungus S7 on Seed Germination of Dendrobium officinale
OBJECTIVE To investigate the effects of carbohydrate components of fungus S7(Tulasnella sp.)on seed germina-tion of Dendrobium ofiicinale.METHODS The polysaccharides of S7 mycelia(MP)and of S7 fermentation broth(FP)were pre-pared by water extraction and alcohol precipitation.MP and FP were hydrolyzed by trifluoroacetic acid to obtain hydrolysates,MPH and FPH.The monosaccharide composition of MP and FP were determined by PMP-HPLC.MP,FP,MPH,FPH,and the unique and mixed monosaccharide of MP were added to water agar(WA)and 1/2 MS media respectively to study the effects of carbohydrate on the seed germination of Dendrobium officinale.RESULTS The germination rate of WA medium group(WACK1)was(88.65± 4.71)%,and seeds germinated to stage 3(protocorm).Compared with WACK1,adding MP and MPH had no significant effect on germination rate(P>0.05),and seeds could germinate to stage 4(having one leaf),while adding FPH could significantly reduce germination rate(P<0.05).MP was composed of glucose,galactose,glucuronic acid,xylose,mannose,fucose,ribose and arabi-nose in a molar ratio of 15.05∶1.00∶0.70∶0.35∶0.30∶0.29∶0.13∶0.13.FP was composed of arabinose,galactose,xylose and glucu-ronic acid in a molar ration of 1.22∶1.00∶0.71∶0.47.Fucose was the unique monosaccharide component of MP.The germination rate and stage 5(having two leaves)germination rate of 1/2 MS medium group(MSCK)was(98.10±0.46)%and(50.97±4.33)%,respectively.Compared with MSCK,adding 0.20-20 mg·L-1 fucose had no significant effect on germination rate(P>0.05),while significantly increased stage 5 germination rate(P<0.05),which was 1.36-1.47 times that of MSCK.CONCLUSION MP and MPH promote the development of protocorm of D.officinale.Fucose is an active component in MPH that could promote the further dif-ferentiation and development of seeds after germination.

Dendrobium officinaleTulasnella sp.mycelia polysaccharidefucoseseed germination

陈彤垚、陈晓梅、杨建文、李媛媛、邱先丹、王爱荣、曾旭、郭顺星

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中国医学科学院北京协和医学院药用植物研究所,教育部中草药物质基础与资源利用重点实验室,北京 100193

铁皮石斛 胶膜菌属 菌丝多糖 岩藻糖 种子萌发

中国医科院医学与健康科技创新工程项目国家自然科学基金

2021-I2M-1-03181573526

2024

中国药学杂志
中国药学会

中国药学杂志

CSTPCD北大核心
影响因子:0.957
ISSN:1001-2494
年,卷(期):2024.59(7)
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