Determination and Preliminary Risk Assessment of 18 Polycyclic Aromatic Hydrocarbons in Seed-Fruit Herbs by Molecular Imprinting Solid Phase Extraction Combined with Gas Chromatography-Mass Spectrometry
OBJECTIVE To research and evaluate risk of 18 polycyclic aromatic hydrocarbons(PAHs)in seed-fruit herbs based on a SPE-isotope dilution GC-MS/MS method for the determination of the residues.METHODS Using isotope as internal standard,the sample was extracted by n-hexane and purified by molecular imprinting solid phase extraction.GC-MS/MS method was used for the as-say.The chromatographic column was DB-17ms(0.25 mm ×30 m,0.25 µm)with temperature programming and MRM detection.The preliminary risk assessment of polycyclic aromatic hydrocarbons(PAHs)in seeds and fruits of traditional Chinese medicine(TCM)was carried out by using toxicity equivalent factor method.RESULTS The calibration curves for the 18 kinds of typical PAHs were linear in the range of 2-50 ng·mL-1.The average recovery rate was in the range of 77.25％-112.32％,with RSD 2.74％-15.89％(n=3).The LOQs were 0.2-1 μg·kg-1 According to the risk assessment results,some varieties may have potential cancer risk.CONCLUSION This method is specific,sensitive,and can be used for residue detection of 18 typical kinds of PAHs in seed-fruit herbs.The residual amount of PAHs in some seeds and fruits of traditional Chinese medicine may have a potential cancer risk,which need attention.
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