首页|9种淫羊藿叶的微性状与显微鉴别研究

9种淫羊藿叶的微性状与显微鉴别研究

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目的 系统研究市售9种淫羊藿叶的微性状及显微特征,归纳其专属且实用的鉴别特征,为市售常见淫羊藿药材的有效鉴别提供参考依据.方法 采用性状鉴别、微性状鉴别及显微鉴别方法,结合景深合成技术,得到9种淫羊藿叶的高清特征图片;将部分特征图进行数字化提取,并用SPSS 26.0软件进行统计学分析.结果 微性状鉴别特征中叶柄毛被、叶缘刺、叶下表面乳突和毛被、叶基部主脉横切面木心数、叶上表面角质层,显微鉴别特征中叶上表皮细胞波状深度、非腺毛、乳突表面及侧面观形态等作为9种淫羊藿叶分类鉴别的专属性特征;不同品种淫羊藿叶边缘刺角度、叶上表皮细胞波状深度占比和乳突密度等特征值的差异具有统计学意义(P<0.05).结论 微性状和显微鉴别均可区分9种淫羊藿叶,微性状特征的量化及其统计学分析弥补了传统经验鉴别受主观因素影响的不足,为市售常见淫羊藿药材流通、检验、临床用药及标准起草等工作提供参考.
Micro-Characteristics and Microscopic Identification of 9 Kinds of Epimedium Leaves
OBJECTIVE To systematically study the micro-characteristics and microscopic characteristics of 9 commercially a-vailable Epimedium leaves,summarize more exclusive and practical features and provide reference for effective identification of Epime-dii Folium on market.METHODS Using the character identification,micro-character identification,microscopic identification and combining with the technique of depth synthesis,high definition feature images of 9 species of Epimedium leaves were obtained.Some of the feature maps were extracted digitally and analyzed by SPSS 26.0 software.RESULTS The micro-characteristics of petiole hair,leaf margin spines,nipple and hairs on lower surface,parenchyma number on the base of leaf main vein transverse section,cuticle on the surface of leaf,as well as the microscopical characters of upper epidermal cell wave depth,non-glandular hair and nipple surface morphology,all of that can be regarded as specific features of 9 species of Epimedium leaves in classification and identification.There was statistically significant difference in the angle of thorns at the edge of leaves,the proportion of wave depth in upper epidermal cells and the density of nipple among all samples(P<0.05).CONCLUSION Nine kinds of Epimedium leaves could be distinguished through the micro-characteristics and micro-identification methods.The quantitative analysis and statistical analysis of micro-property will make up for the deficiency of traditional experience identification by subjective factors.The results of this study will provide a refer-ence for the circulation,inspection,clinical medication and standard drafting and so on.

Epimedium leavesmicro-characteristicsmicro-identificationpolarized lightstatistical analysis

邹立思、易东阳、潘雅楠、林丽群、肖承鸿、裴高升、薛佳、刘训红

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南京中医药大学,南京 210023

重庆三峡医药高等专科学校,重庆 404120

南京传统中医门诊部,南京 210023

贵州中医药大学,贵阳 550025

国药集团同济堂(贵州)制药有限公司,贵阳 550200

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淫羊藿叶 微性状 显微鉴别 偏振光 统计分析

江苏高校品牌专业建设工程项目南京市"科技三峡行"重庆万州区对口支援项目(2023)

PPZY2015A0702023101S-01

2024

中国药学杂志
中国药学会

中国药学杂志

CSTPCD北大核心
影响因子:0.957
ISSN:1001-2494
年,卷(期):2024.59(9)
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