Effect and Mechanism of Atractylodes macrocephala polysaccharide on CUMS Mouse Models
OBJECTIVE To explore the mechanism of Atractylodes macrocephala polysaccharide(AMP)in improving chronic unpredictable mild stress(CUMS)depression in mice by integrating metabolomics technology and gut microbiota analysis.METHODS CUMS depression mouse model was established.Low,medium and high dose AMP treatment were given at 0.062 5,0.125,0.250 g·kg-1,respectively,fluoxetine was given at the dosage of 0.015 g·kg-1,control group and model group were given 0.9％saline solution,and all groups were given at 5 mL·kg-1·d-1ofdrug.The content of 5-hydroxytryptamine(5-HT)in the brain of mice was determined by enzyme-linked immunosorbent assay(ELISA),and the CUMS depression model was tested by combining sucrose preference test(SPT)results.The 16S rDNA amplicon sequencing technology was used to analyze the gut microbiota in the feces of mice in each group.LC-MS technology was used to perform non-targeted metabolomics determination of mouse serum.RESULTS Compared with the control group,the sucrose preference rate of CUMS model mice was significantly lower,and the con-tent of 5-HT in the brain of mice was significantly reduced(P＜0.01),indicating that the CUMS depression model was successfully established.After AMP treatment,the sucrose preference rate and 5-HT content of mice in each group increased(P＜0.01),the gut microbiota of CUMS mice had a regulatory effect,serum metabolites were significantly changed,and 58 metabolites were significantly adjusted.Spearman correlation analysis showed that the changes in gut microbiota were significantly associated with the changes in metabolite levels.CONCLUSION AMP exerts intervention effects on CUMS depression model mice by regulating the stability of gut microbiota,upregulating the F-B ratio,and thereby regulating metabolic pathways.
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