基于LC-MS/MS分析壮药龙钻通痹颗粒的化学成分
Analysis of Chemical Constituents of Zhuang Medicine Longzuan Tongbi Granules by LC-MS/MS
程硕涵 1雷小英 1康雯 1姚兰 1张航 1张青槐 2庞宇舟 2周文斌 3再帕尔·阿不力孜3
作者信息
- 1. 中央民族大学药学院,民族医药教育部重点实验室,北京 100081
- 2. 广西中医药大学,南宁 530001
- 3. 中央民族大学药学院,民族医药教育部重点实验室,北京 100081;中央民族大学生命与环境科学学院,质谱成像与代谢组学国家民委重点实验室,北京 100081
- 折叠
摘要
目的 采用液相色谱-串联质谱联用(LC-MS/MS)技术分析壮药经典方剂龙钻通痹颗粒的化学成分.方法 使用ACQUITY UPLC HSS T3色谱柱(2.1 mm × 100 mm,1.8 pm)及体积分数0.1％甲酸水(A)-0.1％甲酸乙腈(B)流动相系统对供试品溶液进行梯度洗脱,而后在正负离子模式下采集MS/MS数据.结果 通过高分辨质谱数据解析、对照品及数据库比对,同时参考文献已报道的化合物裂解规律,共鉴定出113个化合物,包括生物碱类64个、黄酮类20个、有机酸类12个、香豆素类8个、木脂素类5个及其他类4个,并对其中的109个次生代谢产物进行归属识别.结论 龙钻通痹颗粒物质基础丰富,以生物碱类成分为代表,且飞龙掌血为其重要"主药",以上可为此方剂的药效机制研究与临床合理使用提供科学依据.
Abstract
OBJECTIVE To analyze the chemical components of Longzuan Tongbi Granules,a classical prescription of Zhuang medicine by liquid chromatography-tandem mass spectrometry(LC-MS/MS).METHODS The MS/MS data were collected in positive and negative ion mode after gradient elution of the test solution using an ACQUITY UPLC HSS T3 column(2.1 mm × 100 mm,1.8 μm)and a mobile phase system consisting of 0.1％formic acid water(A)-0.1％formic acid acetonitrile(B).RESULTS A total of 113 compounds,including 64 alkaloids,20 flavonoids,12 organic acids,8 coumarins,5 lignans and 4 others,were identified by high-resolu-tion mass spectrometry data analysis,reference substance and database comparison,while referring to the mass fragmentation pattems of relevant components reported in the literature,and of which 109 secondary metabolites were attributed for recognition.CONCLUSION The material basis of Longzuan Tongbi Granules is rich,represented by alkaloids,and Toddalia asiatica is the important"main drug".This infor-mation can provide a scientific basis for the pharmacodynamic mechanism study and rational clinical use of this formula.
关键词
龙钻通痹颗粒/壮药/生物碱/黄酮/液相色谱-串联质谱联用/质谱裂解规律Key words
Longzuan Tongbi Granule/Zhuang medicine/alkaloid/flavonoid/LC-MS/MS/mass fragmentation pattern引用本文复制引用
基金项目
国家重点研发计划中医药现代化研究重点专项(2017YFC1704000)
出版年
2024
NETL
NSTL
万方数据