Analysis of Chemical Constituents of Zhuang Medicine Longzuan Tongbi Granules by LC-MS/MS
OBJECTIVE To analyze the chemical components of Longzuan Tongbi Granules,a classical prescription of Zhuang medicine by liquid chromatography-tandem mass spectrometry(LC-MS/MS).METHODS The MS/MS data were collected in positive and negative ion mode after gradient elution of the test solution using an ACQUITY UPLC HSS T3 column(2.1 mm × 100 mm,1.8 μm)and a mobile phase system consisting of 0.1％formic acid water(A)-0.1％formic acid acetonitrile(B).RESULTS A total of 113 compounds,including 64 alkaloids,20 flavonoids,12 organic acids,8 coumarins,5 lignans and 4 others,were identified by high-resolu-tion mass spectrometry data analysis,reference substance and database comparison,while referring to the mass fragmentation pattems of relevant components reported in the literature,and of which 109 secondary metabolites were attributed for recognition.CONCLUSION The material basis of Longzuan Tongbi Granules is rich,represented by alkaloids,and Toddalia asiatica is the important"main drug".This infor-mation can provide a scientific basis for the pharmacodynamic mechanism study and rational clinical use of this formula.
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