Effects of Orthogonal Design Optimized Elicitor Combinations on Fucoxanthin Content and Photosynthetic Physiology and Gene Expression of Phaeodactylum Tricornutum
OBJECTIVE To study the effects of different concentrations of photosynthetic induction factor(PIF),methyl jasmonate(MeJA),ceric ammonium sulfate(ACS)and acetylsalicylic acid(ASA)on the accumulation of fucoxanthin in Phaeodacty-lum tricornutum,screen the best elicitor combination for fucoxanthin accumulation,and study the photosynthetic physiology and gene expression changes of fucoxanthin accumulation in Phaeodactylum tricornutum under the treatment of the best inducer combination.METHODS A four-factor,four-level orthogonal test was used to screen the best inducer combination to promote the accumulation of fucoxanthin in Phaeodactylum tricornutum,and the chlorophyll fluorescence parameters of Phaeodactylum tricornutum under the treat-ment of the best elicitor combination were detected by pulse-amplitude-modulation(PAM).The expressions of zds,lcyb,rbcL and psbA were detected by RT-qPCR.RESULTS The results of the orthogonal test showed that MeJA was the most significant factor affecting the accumulation of fucoxanthin in Phaeodactylum tricornutum cells,followed by ASA,and the best elicitor combination to promote the accumulation of fucoxanthin in Phaeodactylum tricornutum was 1μg·L-1 PIF+0.2 mg·L-1 ACS+40 mg·L-1 ASA.The photosyn-thetic physiological study showed that the inductor treatment was able to increase the fucoxanthin content by 104％compared with the control group,which was 21.77 mg·g-1.The results of photosynthetic physiological study showed that the inducer combination signif-icantly increased the rETRmax value(P＜0.05)of Phaeodactylum tricornutum.The expression of rbcL gene was the highest,which was 2.0 times higher than that of the control treatment(P＜0.01).CONCLUSION The expressions of rETRmax,chla content,lcyb,rbcL and psbA genes are highly significantly correlated with zds gene,and the photosynthetic efficiency can be improved by promoting the expression of photosynthetic genes and the relative electron transfer rate of PS Ⅱ,and the expression of genes related to the synthe-sis of lutein can promote the accumulation of fucoxanthin in Phaeodactylum tricornutum.
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