Chemical Identification of TCM Sargassum Based on Characteristic Fatty Acids
OBJECTIVE To study the identification of Sargassum fusiforme and other sargassos(algae from the genus Sargas-sum)by targeted lipidomics combined with chemotaxonomy methods.METHODS Twenty-seven batches of S.fusiforme were collect-ed and the GC-MS fingerprint of fatty acids(FAs)was established.The principal component analysis and orthogonal partial least squares discriminant analysis were used to compare S.fusiforme with other 8 species,total 22 batches of sargassos.Based on the differ-ential FAs between S.fusiforme and other sargassos,combined with the analysis of FA biosynthesis pathway,the Q-marker was screened.RESULTS A total of 29 common FAs were identified by the fingerprint,and the similarity was greater than 0.96.The chemical pattern recognition method can well distinguish S.fusiforme from other 8 species,and the discrimination model established by this method can accurately verify 15 batches of commercial slices.Based on the analysis of differential FAs and FA biosynthesis path-way,monounsaturated fatty acid was found to play an important role in chemical classification.The ratio C20∶1 n-9/C16∶1 n-7 was proposed to be used as a candidate Q-marker for the identification of S.fusiforme and 14 batches of commercial slices can be accurately verified.CONCLUSION This study establishes the identification model of S.fusiforme and other confused sargassos,finds the Q-marker of FAs,and provides a reference for the establishment of the identification method of TCM SARGASSUM.
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