Establishment of UPLC Fingerprint and Effects of Antioxidant Related to Its Spectrum Study of Angelica dahurica cv.Qibaizhi
OBJECTIVE To establish UPLC fingerprint of Angelica dahurica cv.Qibaizhi,identify the compounds of common peaks by UPLC-Q-TOF-MS technology,determine antioxidant activity of each batch and study the relationship between effects of antiox-idant and spectrum of Angelica dahurica cv.Qibaizhi.METHODS The fingerprint of 28 batches of Angelica dahurica cv.Qibaizhi was established by the Similarity Evaluation System of TCM Chromatographic Fingerprint(2012 edition),and the common peaks were identified and evaluated for similarity,and the common peaks were analyzed by UPLC-Q-TOF-MS.The free radical scavenging rate of 1,l-diphenyl-2-trinitrophenylhydrazine(DPPH)was used as antioxidant index to determine its antioxidant activity.Grey correlation degree and partial least squares regression were used to analyze the spectral effect relationship between chemical constituents and antioxidant activities of Angelica dahurica cv.Qibaizhi.RESULTS The fingerprints of 28 batches of Radix Angelica were estab-lished,the similarity was 0.910-0.997.By cluster analysis,the different processing methods can be divided into two categories:sul-fur fumigation and sulphur-free.When the square Euclidean distance is 10,the sulphur-free Angelica dahurica cv.Qibaizhi can be fur-ther divided into two categories,which is consistent with the classification of commodity specifications.A total of 13 common peaks were identified.The common peaks were identified by comparison of standards and UPLC-Q-TOF-MS analysis as quinic acid,dihydroo-roselol,oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,isopimpinellin,oxypeucedanin,imperatorin,phellopterin,cnidilin,isoimperatorin and falcarinolone.By scavenging DPPH free radicals,it was found that 28 batches of Angelica dahurica cv.Qibaizhi had antioxidant capacity.The spectral analysis results showed that the antioxidant capacity was positively related to quinic acid,bergapten,oxypeucedanin,phellopterin,isoimperatorin and falcarinolone.CONCLUSION In this study,a quality evaluation model of Angelica dahurica cv.Qibaizhi based on chemical composition and activity is established,which provides a reference for elu-cidating the antioxidant active ingredients and quality control in Angelica dahurica cv.Qibaizhi.
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