Study on Quality Evaluation of Arnebiae Radix by HPLC Fingerprint and Components Contents Determination
OBJECTIVE To establish the fingerprints of 50 batches of Arnebiae Radix and the methods for determination of the contents of six components.METHODS A high performance liquid chromatographic(HPLC)method was used for the determination of 50 batches of Arnebiae Radix on an Agilent 5 TC-C18(2)column,with acetonitrile-0.05％formic acid aqueous solution as the mo-bile phase,gradient elution at a flow rate of 1 mL·min-1,detection wavelength of 275 nm,and column temperature of 30 ℃.The contents of six naphthoquinone components,i.e.,alkannin,acetylshikonin,deoxyshikonin,β-acetoxyisovalerylshikonin,isobutyryl al-kannin,and β,β'-dimethylacrylalkannin were determined.The fingerprints of the herbs were established by HPLC fingerprinting com-bined with the"Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(Version 2012A)"to evaluate the similar-ity and confirm the common peaks.RESULTS The methodological validation results showed that the peak areas of the six components had good linear relationships in the corresponding concentration ranges,and the contents of the six components ranged from 0.006％-0.237％,0.009％-1.759％,0.001％-0.129％,0.002％-0.625％,0.011％-0.609％,and 0.016％-1.712％,respective-ly.The HPLC fingerprints of Amebiae Radix were established,and a total of nine common peaks were calibrated.The similarity fitting was performed by using the TCM fingerprinting software,and it was found that the similarity of the 50 batches of Arnebiae Radix varied greatly.CONCLUSION The HPLC fingerprint of Arnebiae Radix established in this study and the method of simultaneous determi-nation of the contents of six components are simple,stable,accurate,reliable,and reproducible,which can provide a basis for the quality study of Arnebiae Radix.
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