Preparation of Transferrin Modified IR820 Targeting Liposomes and Its Effect on Photodynamic Therapy of Breast Cancer
OBJECTIVE To prepare and characterize transferrin(Tf)modified liposomes containing photosensitizing agent indo-cyanine green(IR820),and to provide a new idea for the clinical treatment of breast cancer by combining photodynamic therapy(PDT)in 4T1 breast cancer cells and the mouse model of breast cancer in situ.METHODS The liposome loaded with IR820(IR820@Lipo)and the Tf modified liposome loaded with IR820(Tf-IR820@Lipo)were prepared by the film dispersion method,respectively.The encapsulation efficiency was detected by UV method,and the physical and chemical properties were investigated by transmission electron microscope and particle size meter.CCK8 assay was used to evaluate the inhibitory effect of the two drugs on the proliferation of 4TI cells.The intracellular fluorescence intensity of the two drugs under laser irradiation was detected by reactive oxygen species(ROS).In vivo pharmacodynamic study was performed to analyze the anticancer effect and toxic side effects of drugs.RESULTS The average particle size of IR820@Lipo and Tf-IR820@Lipo were(84.30±15.66)and(116.20±14.68)nm,respectively,and the Zeta potential were(-8.21±2.06)and(-5.23±1.19)mV,respectively.Transmission electron microscopy showed that the liposome was spherical and uniformly distributed.The encapsulation efficiency of IR820@Lipo was(94.61±0.67)％,and the drug loading was(8.82±0.92)％.The encapsulation efficiency of Tf-IR820@Lipo was(95.55±0.83)％,and the drug loading was(8.92±1.01)％.Tf-IR820@Lipo could significantly inhibit the proliferation and promote the apoptosis of 4T1 cells under laser irradiation.ROS detection results showed that Tf-IR820@Lipo could significantly enhance the fluorescence intensity of 4T1 cells under laser irradiation.The results of in vivo experiments in tumor-bearing mice showed that the Tf-IR820@Lipo light groups had higher tumor targeting in mice.The pharmacodynamic study in vivo showed that Tf-IR820@Lipo had the strongest inhibitory effect on breast cancer,and did not cause liver and kidney function damage in mice,and had no obvi-ous toxic side effects.CONCLUSION Tf-IR820@Lipo is expected to become a new agent for the treatment of breast cancer due to its high targeting,high efficacy and low toxicity.
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