Analysis of Soluble Sugars in Ophiopohon japonicus Tuberous Roots and Fibrous roots by Gas Chromatog-raphy-Mass Spectrometry
OBJECTIVE To establish a method based on gas chromatography-mass spectrometry(GC-MS)for simultaneous de-termination of a variety of soluble sugars in Ophiopohon japonicus tuberous roots and fibrous roots and to analyze the differences in the soluble sugars by multivariate statistical analysis.METHODS The optimized two-step derivatization method of methoxylation-trimeth-ylsilylation combined with GC-MS was used to determine the soluble sugar components in 18 batches of Ophiopohon japonicus tuberous roots and fibrous roots.And the differences in the components were analyzed by multivariate statistical analysis,and the sweetness val-ues of the tuberous roots and fibrous roots samples were converted according to the contents.RESULTS Eighteen kinds of soluble sugars were identified and 14 kinds of them were quantitatively analyzed in the tuberous roots and fibrous roots of Ophiopohon japonicus.It was found that the ingredients of the soluble sugar components in the tuberous roots and fibrous roots of Ophiopohon japonicus were basi-cally the same,and the average of the total contents of the 14 kinds of soluble sugar components were also basically the same(88.76 mg·g-1 in tuberous roots vs.85.66 mg·g-1 in fibrous roots).However,there were some differences in the contents of the components,and the contents of D-(-)-fructose and in the fibrous roots were significantly higher than those in the tuberous roots,while the contents of D-(+)-sucrose in the tuberous roots were significantly higher than those in the fibrous roots.The results of multivariate statistical analysis showed that tuberous roots and fibrous roots could be significantly differentiated by the determination of soluble sugar fractions.The difference in sweetness values between tuberous roots and fibrous roots was small by conversion.CONCLUSION With high sensitivity,good precision and accuracy,GC-MS is able to distinguish a variety of soluble monosaccharides and disaccharides with simi-lar structures,and realize the accurate characterization and quantification of a variety of soluble sugars in Ophiopohon japonicus,and provi-ding a basis for the study of soluble sugar fractions in different parts of Ophiopohon japonicus and its further development and utilization.
万方数据
维普
