Preparation and Antibacterial Activity of Antimicrobial Peptide PMAP-24KK Hydrogel
OBJECTIVE To prepare hydrogels containing antimicrobial peptide PMAP-24KK and evaluate antibacterial activity.METHODS The antimicrobial peptide PMAP-24KK was combined with sodium alginate,calcium carbonate,and gluconic acid-δ-lac-tone to form the PMAP-24KK hydrogel.Firstly,the formation time,swelling rate,moisture content,water retention rate,and release rate of PMAP-24KK hydrogel were analyzed.Then,the effect of PMAP-24KK hydrogel on the antibacterial activity in vitro,the sustained release,and the antibacterial barrier were determined by drug sensitivity test and skin wound healing in mice was analyzed.RESULTS The results showed that the PMAP-24KK hydrogel could form a gel state with network structure in about 2 min,and the swelling rate and moisture content reached equilibrium at 10 h,which were(158.83±3.26)％and(61.01±2.41)％,respectively.The water retention rate and release rate were about 55％and 81％after 24 h,respectively.The PMAP-24KK hydrogel showed the inhibitory effect on Staphylococcus aureus,Listeria monocytogenes,Salmonella choleraesuis,and Salmonella typhimurium.The sustained-release activity of PMAP-24KK hydrogel had no significant difference in 18 h,and gradually decreased after 18 h to 36 h without antibacterial activity.The PMAP-24KK hydrogel can effectively act as an antibacterial barrier and contribute to wound healing in mouse wound model.CONCLUSION Antimicrobial peptide PMAP-24KK hydrogel with good physicochemical properties and antibacterial activity is successfully prepared in this study,and it has good sustained-release and antibacterial barrier and promote wound healing,the study laid a foundation for further application research of antimicrobial peptide hydrogel.
万方数据
维普
