Viable Bacteria Content and Gene Safety Analysis of Bifidobacterium Microbial Products
OBJECTIVE To investigate the influencing factors and test methods of viable bacterial count in probiotic products of nature Bifidobacterium,and provide reference and suggestions for improving the quality control of such products.METHODS The effects of storage temperature and viable count determination method on viable count were studied,and the pretreatment methods and diluent composition were optimized,and the drug resistance genes and virulence genes of 11 strains contained in four products were analyzed by whole genome sequencing and functional genomics analysis.RESULTS The results showed that temperature had a great influence on the results of viable bacteria count of Bifidobacteria products,and the optimized method of live bacteria count could effectively improve the count results.Several strains of live bacteria products contained drug resistance genes and virulence genes.CONCLUSION It is suggested that we should pay attention to the influence of temperature on products in production storage and circulation.The count method of live bacteria can consider the characteristics of strains and production technology,and optimize the influencing factors such as pre-treatment and diluent.And there is a need for systematic safety assessment of all strains involved in commercially live bacteria product.
万方数据
维普
