OBJECTIVE To prepare bimodal mesoporous silica nanospheres loaded with doxorubicin,and evaluate their adsorp-tion/desorption properties and proliferation inhibition effects on cancer cells.METHODS bimodal mesoporous silica nanospheres(BMSNs)were prepared using sol-gel method and thermal treatment.The nanospheres were analyzed and characterized using scanning electron microscopy,specific surface area and porosity analyzer,Fourier transform infrared spectroscopy,and UV-VIS spectrometer.The adsorption/desorption characteristics of DOX by BMSNs were investigated by adsorption experiment.The proliferation inhibition effects of BMSNs-DOX on MCF-7 cells and A549 cells were detected using CCK8 method.RESULTS Compared with mesoporous MCM-41,which has typical uniform mesopores,BMSNs could provide more favorable matter diffusion and adsorption capacity towards DOX due to their more expansive pore structure.At pH=7.0,BMSNs exhibited a high adsorption capacity for DOX of 91.7 μmol·g-1(equivalent to a drug loading capacity of 5.32％),which was 1.6 times higher than that of MCM-41.In pH=5.0 buffer solution,BMSNs-DOX desorbed(released)DOX molecules with an efficiency of about 96％,in just 30 minutes.BMSNs-DOX exhibited a stronger inhibitory effect than MCM-41-DOX and DOX.At a concentration of 4 μg·mL-1,the survival rate of MCF-7 cells and A549 cells was only 23.67％and 15.79％respectively.CONCLUSION Compared with MCM-41,BMSNs is expected to release DOX to cancer cells more efficiently.BMSNs-DOX exhibits a significant inhibitory effect on MCF-7 cells and A549 cells,suggesting its poten-tial as a drug carrier to enhance the cytotoxicity of chemotherapy drugs on cancer cells.
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