OBJECTIVE To establish a high-throughput qualitative analysis method for the Zhuang medicine Tongfeng Li'an Capsule use by Q-exactive orbitrap HRMS mass spectrometry.METHODS HPLC part on GOLD C18 column(2.1 mm × 100 mm,1.9μm)and the gradient elution was performed with mobile phase consisting of 0.1％formic acid acetonitrile-0.1％formic acid aque-ous solution.Flow velocity was 0.3 mL·min-1,injection volume was 3 μL,column temperature was maintained at 35 ℃,mass spec-trometry part used HESI ion source,Full MS/dd-MS2 mode,ion scan range 100-1 500,vertex excitation for 2-8 s.RESULTS A total of 49 compounds were identified from Tongfeng Li'an Capsule by orbitrap HRMS,including 19 phenylpropanoids,11 terpenes,10 organic acids,3 phenols,2 anthraquinone,2 alkaloids and vitamins,and 2 sugars and glycols.Among them,46 compounds were identified for the first time from Tongfeng Li'an Capsule.CONCLUSION Data for high-resolution gather with orbitrap HRMS method was the first time comprehensive qualitative characterization conducted on Tongfeng Li'an Capsule,This provides a material basis and new scientific research basis and reference for its multi-component analysis application,quality standard improvement,and pharmaco-logical research.
关键词
静电场轨道阱/高分辨质谱/痛风立安胶囊/壮药/定性/苯丙素类/萜类/有机酸类
Key words
Q-exactive orbitrap/HRMS mass spectrometry/Tongfeng Li'an Capsule/Zhuang medicine/qualitative analysis/phenylpropanoid/terpene/organic acid
维普
万方数据