首页|静电场轨道阱高分辨质谱技术在壮药痛风立安胶囊中的定性应用

静电场轨道阱高分辨质谱技术在壮药痛风立安胶囊中的定性应用

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目的 利用静电场轨道阱高分辨质谱技术(Orbitrap HRMS)，建立壮药制剂痛风立安胶囊的高通量定性筛查方法。方法 液相部分采用GOLD C18(2。1 mm × 100 mm，1。9 μm)色谱柱，以体积分数0。1％甲酸水-0。1％甲酸乙腈为流动相梯度洗脱，流速0。3 mL·min-1，进样量3 μL;柱温35 ℃;质谱部分采用HESI离子源，Full MS/dd-MS2模式，离子扫描范围100～1 500，顶点激发2～8 s。结果 通过Orbitrap HRMS的高通量筛查方法共筛选出49个化合物，包括苯丙素类19个，萜类11个，有机酸类10个，酚类3个，蒽醌类2个，生物碱及维生素类2个，糖及糖醇类2个，其中有46个化合物为从痛风立安胶囊中首次鉴定得到。结论 通过Orbitrap HRMS技术采集的高分辨数据建立的方法，首次对痛风立安胶囊进行了全面的定性表征，为其多组分分析应用及质量标准提升、药效研究提供了物质基础及新的科学研究依据和参考。
Qualitative Analysis of Zhuang Medicine Tongfeng Li'an Capsule by Q-Exactive Orbitrap HRMS Mass Spectrometry
OBJECTIVE To establish a high-throughput qualitative analysis method for the Zhuang medicine Tongfeng Li'an Capsule use by Q-exactive orbitrap HRMS mass spectrometry.METHODS HPLC part on GOLD C18 column(2.1 mm × 100 mm,1.9μm)and the gradient elution was performed with mobile phase consisting of 0.1％formic acid acetonitrile-0.1％formic acid aque-ous solution.Flow velocity was 0.3 mL·min-1,injection volume was 3 μL,column temperature was maintained at 35 ℃,mass spec-trometry part used HESI ion source,Full MS/dd-MS2 mode,ion scan range 100-1 500,vertex excitation for 2-8 s.RESULTS A total of 49 compounds were identified from Tongfeng Li'an Capsule by orbitrap HRMS,including 19 phenylpropanoids,11 terpenes,10 organic acids,3 phenols,2 anthraquinone,2 alkaloids and vitamins,and 2 sugars and glycols.Among them,46 compounds were identified for the first time from Tongfeng Li'an Capsule.CONCLUSION Data for high-resolution gather with orbitrap HRMS method was the first time comprehensive qualitative characterization conducted on Tongfeng Li'an Capsule,This provides a material basis and new scientific research basis and reference for its multi-component analysis application,quality standard improvement,and pharmaco-logical research.

Q-exactive orbitrapHRMS mass spectrometryTongfeng Li'an CapsuleZhuang medicinequalitative analysisphenylpropanoidterpeneorganic acid

廖强、陈松、李运容、黎丽、韦环

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广西壮族自治区食品药品审评查验中心,南宁 530022

广西国际壮医医院,南宁 530200

广西-东盟食品检验检测中心,南宁 530022

静电场轨道阱 高分辨质谱 痛风立安胶囊 壮药 定性 苯丙素类 萜类 有机酸类

黄瑞松全国名老中医药专家传承工作室科研项目资助广西中医药大学校级科研项目资助广西国际壮医医院引进人才科研启动项目资助国家中医药管理局高水平中医药重点学科建设项目资助广西壮族自治区中医药管理局中药壮瑶药医院制剂质量提升项目资助

2023MS040GZ2023RC007zyyzdxk-2023165GZZJ202014

2024

10.11669/cpj.2024.14.010

中国药学杂志
中国药学会

中国药学杂志

CSTPCD北大核心
影响因子：0.957
ISSN：1001-2494
年,卷(期)：2024.59(14)