Development of ISSR-SCAR Molecular Markers for Original Plants and Related Plants of Rabdosia Herba
OBJECTIVE To study and establish sequence characterized amplifiedregion(SCAR)molecular marker for identifying the original plants and related plants of Rabdosia Herba,and provide a new molecular identification method for the original plants of Rabdosia Herba.METHODS A total of 29 populations of the original plants and related plants of Rabdosia Herba were collected,and inter-simple sequence repeat(ISSR)molecular marker was used to perform PCR amplification on the genomic DNA of the representative population of Rabdosia Herba to obtain ISSR-specific segments.After the ISSR-specific fragments were recovered,purified,cloned and sequenced,SCAR primers were designed based on the sequencing results,which were directly used for the efficient identification of the original plants of Rabdosia Herba.RESULTS Fourteen pairs of ISSR primers were used to perform PCR amplification on 18 representa-tive samples of original plants and related plants of Rabdosia Herba,and 4 specific segments were obtained and successfully transformed into ISSR-SCAR molecular markers.These molecular markers can be used to specifically identify the Rabdosia lophanthoides(Buch.-Ham.ex D.Don)H.Hara,Rabdosia stracheyi(Benth.Ex Hook.f.)Hara,Rabdosia serra(Maxim.)H.Hara,and Rabdo-sia nervosa(Hemsl.)C.Y.Wu et H.W.Li,respectively.CONCLUSION The established ISSR-SCAR molecular markers produce clear and bright bands with stable results,which can accurately and rapidly identify the original plants and related plants of Rabdosia Herba,providing a new method for the identification of the original plants of Rabdosia Herba.
Rabdosia Herba original plantmolecular markerISSRspecific segment
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