UPLC-MS/MS Multi-Index Component Analysis of Red Ginseng Combined with Chemometrics and EW-TOPSIS Method to Evaluate the Quality of Shenmai Granules
OBJECTIVE To establish a UPLC-MS/MS method for simultaneous determination of 14 components in Shenmai gran-ules,and analyze the quality differences of red ginseng feed from different manufacturers.METHODS Shimadzu Shim-pack gist C18(2.1 mm × 100 mm,2 μm)chromatographic column with acetonitrile-water as the mobile phase were used to determine the contents of 14 saponin components in 71 batches of Shenmai Granules.And then analyzed the main material basis affecting the difference in red ginseng feeding in Shenmai granules combined with chemometrics and evaluated the quality of red ginseng feeding in Shenmai granules from different manufacturers combined with EW-TOPSIS.RESULTS The concentrations of 14 components had a good linear relation-ship(r≥0.998 5);and the average recovery rate was 95.6％-105.0％(RSD＜4.7％).According to the analysis of chemometrics and EW-TOPSIS,the samples produced by the three manufacturers were grouped into three categories,among which the quality of red ginseng feeding by manufacturer B was generally good,and the red ginseng feeding by manufacturer C was adulterated.CONCLUSION This method is specific,sensitive,efficient,and reliable,and can be used for the quality control of Shenmai granules.
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