OBJECTIVE To screen the commonly used pesticides in Dendrobium officinale to understand their pesticide residues,and explore the establishment of a method for determining the transformation of pesticide residues in Dendrobium officinale and the development of limit standards in accordance with the requirements of GB 2763 Food Safety Standards for Maximum Residue Limits of Pesticides in Food and the Chinese Pharmacopoeia.METHODS LC-MS/MS methods were used to screen and risk assess 6 transfor-mation pesticides in 53 batches of Dendrobium officinale,and the pesticide registration status and the maximum residue limit standards for Dendrobium officinale(dry)in GB 2763 were used to determine the indicators for the transformation of pesticides.RESULTS The pesticides in 53 batches of Dendrobium officinale were analyzed.The transformation pesticide were confirmed in accordance with GB 2763 and the requirements of the Chinese Pharmacopoeia 2020 Edition,and a method for determining the transformation of pesti-cide residues in Dendrobium officinale was established,along with the development of limit standards.CONCLUSION This study screened 40 commonly used pesticides and 6 transformation in 53 batches of Dendrobium officinale,established a method for determi-ning the transformation of pesticide residues in accordance with GB 2763,and developed limit standards.This study is helpful for un-derstanding the risk of pesticide residues in Dendrobium officinale,regulating the use of pesticides during its cultivation process,and improving its quality standards.