Research Progress in the Use of Cell Line and Organoid Models for Studying Inflammatory Bowel Disease
It is important to select a suitable in vitro experimental model to study the pathology and pharmacological mechanisms of inflammatory bowel disease(IBD),thereby developing advanced therapeutic drugs.Immortalized cell lines,as a classic in vitro mod-el,offer several advantages in the research and drug evaluation of IBD,such as high efficiency,low cost,simple operation and intui-tive experimental results.However,these models cannot recapitulate the multicellular composition and intercellular interaction of intes-tinal tissue in vivo and may lose genetic characteristics after multiple passages in vitro.The development of organoids and organs-on-a-chip has promoted the technological innovation of in vitro models by significantly improving our capability to simulate the architecture and function of IBD.The enhanced reproducibility of the organoid model to the microenvironment of the source tissue also confers im-proved predictive capability for patient treatment response.This paper reviews the current research status of these models by discussing their characteristics,advantages,disadvantages,and their applications in evaluation of IBD therapeutic drugs and development of ad-vanced therapeutic drugs,as well as in the exploration of pharmacological mechanisms.
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