Single-Cell Sequencing Reveals the Senescence State of Stem Cells in Ulcerative Colitis
OBJECTIVE To analyze the dynamic changes of cell types and protein expression during the occurrence and develop-ment of ulcerative colitis.METHODS Single-cell sequencing technology was used to analyze the intestinal tissues of ulcerative colitis model mice and normal mice.After quality control,a total of 58 714 cells were used to construct a colitis dataset for downstream analy-sis.Then,the changes of epithelial compartments in the process of colitis occurrence and development were analyzed by means of dimensionality reduction clustering,GO analysis and Monocle quasi-time analysis.RESULTS Compared with the normal samples,the interaction between cell subsets in colitis samples changed significantly.The analysis revealed that most epithelial cell subtypes were destroyed and reduced in number during the colitis phase.The Wnt pathway was inhibited,which is important for maintaining stem cell function.The Ppia-Bsg ligand receptor pair was highly expressed in normal samples.CONCLUSION The depletion of Ppia secreted by stromal cells in the inflammatory state leads to stem cell senescence,which ultimately disrupts the homeostasis of epithelial cells and aggravates colitis.
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