Analysis of Chemical Variability on Different Processed Products of Polygonati Rhizoma Produced in Shaanxi Province Based on Plant Metabolomics
OBJECTIVE To analyze the difference of chemical components of different processed products of Polygonati Rhizoma produced in Shaanxi Province.METHODS Ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)was developed to determine the chemical components of different processed products of Polygonati Rhizoma produced in Shaanxi Province.The data was analyzed by principal component analysis(PCA)and orthogonal par-tial least squares discriminant analysis(OPLS-DA).The structures of chemical markers of different processed products of the plants were identified based on accurate primary mass spectrometry and secondary mass spectrometry fragment ion,combined with the reference map,software database searching and related literature.RESULTS A total of 45 components were identified,there were 23 chemical compo-nents with significant differences distinguished by the method of OPLS-DA,including 13 amino acids,5 alkaloids,2 saccharides,1 cou-marin,1 saponin,1 organic acids.CONCLUSION There are some chemical differences among the three products.This study can pro-vide material basis for the quality control and pharmacodynamic research of processed products of Polygonati Rhizoma.
万方数据
维普
