Quantitative Determination of Multiple Components in Nardostachys jatamansi DC.Based on"Spider-web"Model and UHPLC-Q-Exactive-Orbitrap-MS
OBJECTIVE To establish a UHPLC-Q-Exactive-Orbitrap-MS method to determine the contents of six sesquiterpenes in Nardostachys jatamansi DC.based on the'spider web'model.METHODS The extraction methods of six sesquiterpenes in Nardostachys jatamansi DC.were studied and determined by cobweb mode.Waters CORTECSTM C18 chromatographic column(4.6 mm × 150 mm,2.7μm)was used.Acetonitrile was used as mobile phase A,and 0.05％formic acid solution containing 5 mmol·L-1 ammonium formate was used as mobile phase B.Gradient elution was conducted at a flow rate of 0.5 mL·min-1.The column temperature was maintained at 35 ℃.Detection was carried out in electrospray positive ion mode.RESULTS The results of spider web showed that the optimum extraction con-ditions were methanol extraction:solid-liquid=1:12.5,heating reflux for 30 min.Deoxyeugenol A,nardosinonediol,nardosinone A,axinysone B,nardosinone and nardosinone H had good linear relationship with the peak area integral value in the range of 0.386 4-1.425 6,0.366 3-2.424 5,0.462 1-1.458 8,0.639 7-1.783 2,3.542 5-14.657 6 and 0.432 7-1.439 3 μg·mL-1,respec-tively,and the correlation coefficients(r)were not less than 0.999 3.The average recoveries of the six components were 98.39％,100.05％,100.35％,97.13％,102.84％and 97.15％,respectively.The results were analyzed and evaluated by clustering and partial least squares(PLS-DA).The results showed that the potential differential markers of Nardostachys jatamansi DC were nardosinone,nardosinone A and nardosinone H.CONCLUSION The method established in this study can realize the rapid and accurate quantitative analysis of six sesquiterpenes in Nardostachys jatamansi DC,which can provide reference for the quality evaluation of Nardostachys jatamansi DC.
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