Advances in the Application of Microscale Thermophoresis in Drug Target Discovery
The discovery of drug targets is crucial for understanding the mechanism of drug action and facilitating the development of novel drug therapeutics.Microscale thermophoresis(MST),renowned for its minimal sample consumption,rapid detection speed,high sensitivity,and the absence of the need for protein immobilization,has emerged as a pivotal tool in drug target research.This arti-cle outlines the strategies for identifying drug targets,with a focus on elucidating the principles of MST and its application in drug target discovery.Furthermore,it summarizes the promising prospects of MST in this field.The applications of MST are found in both forward and reverse strategies for target discovery,enabling the identification of target proteins for compounds,single herbal ingredients,and compound prescriptions,as well as facilitating drug screening.Additionally,the integration of MST with other molecular interaction techniques,such as surface plasmon resonance(SPR),provides a potent arsenal for studying the interactions between drugs and their targets.Despite certain limitations,MST holds immense potential for application in the realms of novel drug development and drug mechanism research.
NETL
NSTL
万方数据
