基于化学标识物的特征多肽技术在鹿源药材区分与鉴别中的应用研究
Application Research of Characteristic Polypeptide Technology Based on Chemical Markers in Distiguish-ment and Identification of Deer-derived Medicinal Materials
祝玉卓 1薛菲 2焦阳 1崔伟亮 2陈娟 1汪冰 1林永强1
作者信息
- 1. 山东中医药大学,济南 250355;山东省食品药品检验研究院,国家药监局胶类产品质量评价重点实验室,山东省中药标准创新与质量评价工程实验室,中药配方颗粒共性技术山东省工程研究中心,济南 250101
- 2. 山东省食品药品检验研究院,国家药监局胶类产品质量评价重点实验室,山东省中药标准创新与质量评价工程实验室,中药配方颗粒共性技术山东省工程研究中心,济南 250101
- 折叠
摘要
目的 基于化学标识物概念,通过特征多肽分析方法,实现对鹿茸、鹿角、鹿角霜和鹿皮4种不同部位来源的鹿源药材的精准区分.方法 比较了 2种提取溶剂体系对4种不同部位来源的鹿源药材提取效率,优化样品最佳酶解条件,形成高效、简便的样品前处理流程.结合化学标识物理念和特征多肽分析方法,采用液相色谱-三重四极杆质谱及质谱多反应监测技术(MRM),建立一套针对4种不同部位鹿源药材的专属性鉴别方法,并进行方法学实验.结果 优化后的样品前处理方法提取效率高、酶解效果好,24个批次的样品均成功检测到相应的特征多肽离子对.结论 本研究建立的鹿源药材区分与鉴别方法,具有高专属性、灵敏度和耐用性,能够有效用于鹿源药材的质量评价,为确保和提升鹿源药材的质量提供参考.
Abstract
OBJECTIVE To accurately distinguish deer-derived medicinal materials from four different position:Cervi Comu Pantotrichum,Cervi Cornu,Cervi Cornu Degelatinatum and Deer hide.METHODS The extraction efficiency of two extraction solvent systems for four different kinds of deer-derived medicinal materials was compared,and the optimum enzymatic hydrolysis conditions of the samples were optimized to form an efficient and simple sample pretreatment process.A specific analysis method,combining chemical markers and characteristic polypeptides was established to identify four different position of deer-derived medicinal materials by using liquid chromatography-triple quadrupole mass spectrometry and mass spectrometry multi-reaction monitoring technology(MRM).And methodological experiments were conducted as part of this analysis method.RESULTS The optimized sample pretreatment method with high extraction efficiency and good enzymatic effect was successfully applied,and the corresponding characteristic polypeptide ion pairs were successfully detected in 24 batches of samples.CONCLUSION The distinction and identification method with high-level specificity,sensitivity and robustness of deer-derived medicinal materials were established.The innovative analysis method can be effectively used to evaluate the quality of deer-derived medicinal materials,which provides a reference for ensuring and improving the quality of deer-derived medicinal materials.
关键词
化学标识物/特征多肽/鹿源药材/液质联用技术/多反应监测Key words
chemical marker/characteristic polypeptide/deer-derived medicinal material/LC-MS/MRM引用本文复制引用
出版年
2024
NETL
NSTL
万方数据