OBJECTIVE To determine the antioxidant activities of Gossampim Flos waterextracts,establish UPLC fingerprints and study the spectral effect relationship.METHODS Based on the scavenging rates of 2,2-diazo-di(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS)and 1,1-diphenyl-2-picrohydrazyl radical(DPPH),the fingerprint of different parts(petal,calyx and stamen)of 12 batches of Gossampim Flos were established by UPLC method,and similarity analysis were carried out.Grey corre-lation analysis(GRA),Spearman analysis,stepwise regression analysis and partial least squares regression(PLSR)analysis were used to study the relationship between antioxidant spectrum effect RESULTS Eight chromatographic peaks were identified by fingerprint identification of water extracts from different parts of 12 batches of Gossampim Flos.Mangiferin,quercetin,rutin,vitexin and other compounds were the main active components of Gossampim Flos and its different parts for antioxidant activities.CONCLUSION The antioxidant activity of Gossampim Flos and its different parts are the result of the combined effect of multiple components.This study can provide a comprehensive reference for the basis and quality control of antioxidant active substances of Gossampim Flos and its different parts.
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