OBJECTIVE To predict the potential quality marker (Q-Marker)components of Suanzaoren Decoction based on the prototype chemical composition of in vivo by using network pharmacology.METHODS Firstly,the plasma samples and urine samples of rats were collected within 4 h and 24 h after intragastric administration of Suanzaoren Decoction,respectively.Then,based on the chemical components found in vivo,the key action targets and key pathway were obtained by network pharmacology,and the network map of"component-target-pathway"was carried out to predict the quality markers of Suanzaoren Decoction against insomnia,anxiety and depression.RESULTS A total of 20 prototype compounds were detected in rats.The 23 core targets were predicted and 14 prototype components associated with pharmacodynamic efficacy were screened through network pharmacology,the 14 components including jujuboside A,enoxolone,ferulic acid,magnoflorine,liquiritigenin,isoliquiritigenin,poricoic acid A,spinosin,coclaurine,timosaponin BⅡ,glycyrrhizic acid,jujuboside B,liquiritinand senkyunolide A played multiple targets and multiple pathways to regulate insomnia,anxiety,depression.CONCLUSION It is predicted that the 14 chemical components are considered as potential quality markers of Suanzaoren Decoction by analyzing prototype chemical composition in vivo combined with network pharmacology.The results can provide reference for the quality control of Suanzaoren Decoction,and lay a foundation for elucidating the mechanism of its pharmacodynamic substances.
Suanzaoren Decoctionchemical component in vivohigh performance liquid chromatography quadrupole time-of-flight tandem mass spectrometrynetwork pharmacologyQ-Marker
NETL
NSTL
万方数据
