Preparation and Performance Study of Puerarin Daidzein Inclusion Complex Nanocrystals
OBJECTIVE To prepare puerarin/daidzein porous nanocrystals using polyethyleneglycol(PEG)and chitosan(CS)as carrier aterialsand evaluate their physicochemical properties and in vitro characteristics.METHODS The drug loading rate and entrapment rate were used as indicators to optimize the prescription,and the insoluble drug puerarin-daidzein was encapsulated in the form of inclusion compound by two-step method β-CD-puerarin-daidzein-PEG-CS nanocrystals.The inclusion degree of puerarin-daid-zein nanocrystals was determined by Fourier transform infrared spectroscopy,thermogravimetry,X-ray diffraction,scanning electron microscope,antioxidant and bacteriostatic tests to verify the feasibility of the preparation method.And investigate its drug release behavior in simulated gastrointestinal fluid and gastric environment,and fit the drug release model.RESULTS The inclusion compound prepared by saturated aqueous solution method was the best when the volume fraction of inclusion compound was 1.000％acetic acid.Its cumulative release rate is about 90％,drug loading and inclusion rate are(26.13±0.74)％and(78.39±2.23)％,respectively.Puerarin-daidzein nanocrystals have many and dense pore diameters,and have good antioxidant performance.Obvious inhibition zone against Escherichia coli,Staphylococcus aureus,and Pseudomonas aeruginosa can be observed,showing good antibacte-rial performance.CONCLUSION Puerarin-Daidzein porous nanocrystals can significantly improve the dissolution of insoluble drug daidzein,which lays a foundation for its application in the field of biomedicine.
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